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B. pi. β-glucuronidase variant

B. pi. β-glucuronidase variant

Regular price $89.00 USD
Regular price Sale price $89.00 USD
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Overview
Recombinantly expressed beta-glucuronidase variant sourced from Brachyspira pilosicoli hydrolyzes the glucuronic acid conjugate from phase II and other metabolites. The engineered B. pilosicoli variant has up to 50-fold increased activity on several recalcitrant substrates relative to the wild-type enzyme1. This enzyme has broad activity over a wide pH range (5.5 to 8.0) and is ideal for metabolomics research of known or unknown glucuronidated analytes.

PRODUCT SPECIFICATIONS:

Protein Concentration: 2.0 - 2.1 mg/mL
Enzyme Activity: ≥ 5 U/mg*
Guaranteed Shelf Life: 12 months**
Storage Temperature: 2-8°C

*Enzyme activity was determined using the generic beta-glucuronidase substrate 4-methylumbelliferone (4-MUG). One unit activity is defined as 1.0 µmole 4-MUG hydrolyzed per minute at pH 6.5 and 24 °C (λexcitation: 365 nm and λemission: 445 nm)
**Enzyme solution is stable for at least 12 months at 2-8 °C when stored in its original packaging.

COA and Safety Information

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